Supplemental Data Resource A Human Protein-Protein Interaction Network: A Resource for Annotating the Proteome
نویسندگان
چکیده
Verification of Y2H Interactions by Coimmunoprecipitation and Pull-Down Assays To evaluate the quality of the Y2H data, a representative sample of Y2H interactions was randomly selected for verification assays, because interactions recapitulated independently are unlikely to be experimental false positives (Goehler et al., 2004 ̧ Tewari, 2004 #137). To verify Y2H interactions, we used in vitro pull down assays (Goehler et al., 2004) and a novel membrane coimmunprecipitation assay.
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